Journal: bioRxiv

Article Title: Rhamnolipids from Pseudomonas aeruginosa Disperse the Biofilms of Sulfate-Reducing Bacteria

doi: 10.1101/344150

Figure Lengend Snippet: D. vulgaris biofilms were formed on an 8 chamber cell culture slide for h in modified Baar’s medium, and supernatants were concentrated to 4x and contacted with D. vulgaris biofilms for 0.33 h. Supernatants of (A) P. aeruginosa PA14, (B) P. aeruginosa PA14 rhlA , and (C) PBS buffer. Scale bar is 50 µm.

Article Snippet: Diluted overnight cultures (300 μL) at an initial turbidity at 600 nm of 0.1 were inoculated into an 8 chamber cell culture slide (Dot Scientific, Inc, Burton, MI) with modified Baar’s medium, and the bacteria were cultured for 48 hours at 30°C without shaking.

Techniques: Cell Culture, Modification